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. 2017 Jun 9;8:256. doi: 10.3389/fneur.2017.00256

Figure 2.

Figure 2

Sodium phenylbutyrate (SPB) dose-dependently increases the DJ-1 protein level and rescues the DJ-1 decrease induced by oxygen glucose deprivation (OGD) in SH-SY5Y cells. (A) Western blot analysis of the DJ-1 level in SH-SY5Y cells treated with different concentrations of SPB. Cells were treated with SPB for 24 h and were harvested for analysis. Quantitation data were shown as the ratio of the density; *p < 0.05, **p < 0.01, ***p < 0.001, one-way analysis of variance (ANOVA), n = 3. Western blot (B) and immunofluorescent analyses (C) of the DJ-1 level in SH-SY5Y cells treated with SPB and OGD. Quantitation data of the western blotting assay are shown below in (B) as the ratio of the density; **p < 0.01, vs. the control group; #p < 0.05, vs. OGD without the SPB group, one-way ANOVA, n = 3. For immunocytochemistry (C), the bar represents for 100 µm, and the insets represent the boxed areas (red: DJ-1; blue: DAPI).