FIG. 5.

(A) Release of soluble vWF from uninfected and HCMV-infected HPAEC, as measured by an ELISA. Infected cells secreted 2.5-fold more vWF than uninfected cells at 5 and 7 dpi. (B and C) Secretion of serotonin (B) and soluble P-selectin (C) from platelets incubated with uninfected and HCMV-infected cells at 1, 3, 5, and 7 dpi. High levels of P-selectin and serotonin were observed to be secreted from platelets incubated with HCMV-infected cells at 5 and 7 dpi compared to platelets incubated with uninfected cells. (D) Expression of P-selectin on platelets incubated with nonfiltered or filtered supernatants obtained from HCMV-infected cells. Expression was twofold higher for HCMV-infected cells than for uninfected cells. OD, optical density. Data represent the mean ± SEM for three experiments.