Figure 1. γδT17 cells downregulate CCR6 upon activation.

(a) Representative flow cytometry of CCR6 and CCR2 expression in skin-draining lymph nodes (sLN) and dermal CD3⁺TCR-γδ⁺IL-17A-YFP⁺ γδT17 cells from Il17a^Cre × Rosa26^eYFP mice (n=3). (b) Ex vivo transwell chemotaxis of Il17a^Cre × Rosa26^eYFPsplenic IL-17A^+/− γδ T cells to CCL20 and CCL2 (n=3). (c) Representative flow cytometry of CD45⁺ γδT17 cells from organs of naïve Il17a^Cre × Rosa26^eYFP mice (n=3). mLN, mesenteric lymph node; PP, Peyer's patches; siLPL, small intestinal lamina propria lymphocytes. (d) Representative flow cytometry and quantitation of CCR6 and CCR2 expression by γδT17 cells from organs of Il17a^Cre × Rosa26^eYFP mice either naïve (n=6) or at experimental autoimmune encephalomyelitis (EAE) onset (n=7) or peak (n=5). CNS, central nervous system; iLN, inguinal lymph node; ND, not detected. (e) Representative flow cytometry and quantitation of CCR6 expression by γδT17 cells from wild type (WT) mice given BrdU at d3 post-immunization for EAE, and analysed at d8 (n=4). (f) Representative flow cytometry and frequency of CCR6 and CCR2 expression by γδT17 cells from Il17a^Cre × Rosa26^eYFP lymphocytes cultured with indicated stimuli for 72 h (n=5). See also Supplementary Figs 1 and 2. Mean±s.e.m. (a–c) Representative of two experiments. (d,f) Pooled from two experiments. (e) Paired two-tailed Student's t-test, (f) one-way paired ANOVA with Dunnett's multiple comparisons test relative to unstimulated control. *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001.