FIG. 7.

β-Chemokine neutralization partially reduces the enhanced inhibition of HIV-1 on PBMCs. Goat anti-MIP-1α, anti-MIP-1β, and anti-RANTES antibodies or normal goat IgG was added to CD4⁺ lymphocytes or PBMC target cells immediately prior to infection with 4,500 TCID₅₀ of HIV_92US657 and treatment with anti-HIV or control IgG. All three anti-β-chemokine antibodies were used in combination at final concentrations of 100 μg/ml (anti-MIP-1α and anti-MIP-1β) and 20 μg/ml (RANTES); fresh goat antibodies were added after the 4-day washout (which removed the virus inoculum and the treatment IgG). p24 in supernatant fluid was measured 3 days later (7 days after infection), and the percentage of inhibition was calculated as described in Materials and Methods. Data represent the mean plus SD from two separate experiments, each run in triplicate.