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. 2005 Feb;79(4):2393–2403. doi: 10.1128/JVI.79.4.2393-2403.2005

FIG. 7.

FIG. 7.

Effect of UTP analogs on norovirus and FCV polymerase activity. (A) The norovirus His-Pro−Pol at 1 μM was incubated for 20 min at 30°C with increasing concentrations (1, 5, 50, 100, and 200 μM) of UTP analogs. The modified NTPs are 5-iodo-UTP (compound A), pseudo-UTP (compound B), 5-methyl-UTP (compound C), 2-thio-UTP (compound D), and 6-aza-UTP (compound E). The relative quantities of incorporated UMP (ordinate) are given in percentages. The data (shown as percentages of UMP incorporation) were normalized based upon the UMP incorporated by His-Pro−Pol (100%) in the absence of UTP analog. (B) The recombinant FCV ProM-Pol at 1 μM was assayed as described in the legend for panel A except that the reaction mixture was incubated for 2 min. Code names of the compounds are identical to those in panel A. The data (shown as percentages of UMP incorporation) are normalized based upon the UMP incorporated by FCV rProM-Pol (100%) in the absence of UTP analog.