FIG. 1.

XhoI RFLP analysis for detection of different HDV genotypes in a mixed infection. (A) XhoI restriction sites in the amplified sequences of different genotypes. The PCR products of genotypes I and IIb each have a single XhoI site, at nt 1274 and 971, respectively. Genotype II PCR products contain both of these XhoI sites, whereas genotype III contains no XhoI site in this region. (B) The XhoI-cleaved PCR products of HDV genomes were electrophoresed on a 3% agarose gel and stained with ethidium bromide. Lanes: M, 100-bp-ladder molecular size markers; 1 and 10, undigested PCR products; 2 and 9, digested genotype I PCR products; 3 and 8, digested genotype IIb PCR products; 4, digested PCR products of mixed in vitro-transcribed genotype I and IIb RNAs; 5, digested PCR products of mixed total cellular RNAs extracted from cells transfected separately with genotype I and IIb RNAs; 6, a complex RFLP pattern obtained from the HDV patient; 7, digested genotype II PCR products.