FIG. 2.

siRNA-4 interferes with CVB3 replication in HeLa cells. (a and b) Dose-dependent inhibition of CVB3 production by siRNA-4. HeLa cells were transfected with siRNA-4 at a final concentration as indicated and followed by infection with a CVB3 MOI of 10 for 8 h. Supernatants were used for viral plaque assay (a), and cell lysates were collected for viral VP1 detection by Western blotting (b). β-Actin served as the loading control. (c and d) Time course of inhibition of CVB3 replication by siRNA-4. Cells were transfected with siRNA-4 at a final concentration of 300 nM and followed by CVB3 infection at an MOI of 10. Supernatants and cell lysates were collected at the indicated time points for plaque assay (c) and Western blotting (d), respectively. Data shown are representatives of two independent experiments.