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Schematic of cell synchronization protocol. Asynchronously dividing BCBL-1 cells were arrested in G0 by 24 h of incubation in SFM. Following the reintroduction of serum, TPA was added to induce lytic replication at 0, 6, 16, and 24 h after release from G0 block (asterisks). Parallel unsynchronized BCBL-1 cells, TPA treated or untreated, were prepared as controls.
