Table 2.
Primers used in PCR for detection of virulence genes in antimicrobial resistant non-typhoidal Salmonella
| Primer | Broad function (gene function) | Sequence (5’ to 3’) | Referance |
|---|---|---|---|
| invA | Host recognition/invasion (type III secretion system appratus) | F-CTGGCGGTGGGTTTTGTTGTCTTCTCTATT | Skyberg et al., (2006) [34] |
| R-AGTTTCTCCCCCTCTTCATGCGTTACCC | |||
| orgA | Host recognition/invasion (type III secretion system appratus) | F-TTTTTGGCAATGCATCAGGGAACA | Skyberg et al., (2006) [34] |
| R-GGCGAAAGCGGGGACGGTATT | |||
| prgH | Host recognition/invasion (type III secretion system appratus) | F-GCCCGAGCAGCCTGAGAAGTTAGAAA | Skyberg et al., (2006) [34] |
| R-TGAAATGAGCGCCCCTTGAGCCAGTC | |||
| sopB | Host recognition/invasion (type III secretion system appratus) | F-CGGACCGGCCAGCAACAAAACAAGAAGAAG | Skyberg et al., (2006) [34] |
| R-TAGTGATGCCCGTTATGCGTGAGTGTATT | |||
| tolC | Host recognition/invasion (outer membrane channel protein) | F-TACCCAGGCGCAAAAAGAGGCTATC | Skyberg et al., (2006) [34] |
| R-CCGCGTTATCCAGGTTGTTGC | |||
| sopE | Host recognition/invasion (invasion-associated secreted protein) | F-CATAGCGCCTTTTCTTCAGG | Suez et al., (2013) [35] |
| R-ATGCCTGCTGATGTTGATTG | |||
| sseI | Host recognition/invasion (type III secretion system effector protein) | F-CGCCATCATCAGTAACCGCC | Suez et al., (2013) [35] |
| R-CTGCTGACCACATCCTCCC | |||
| ssek3 | Host recognition/invasion (type III secretion system effector protein) | F-TATCAATCTCAAATCATGG | Suez et al., (2013) [35] |
| R-CGCGTTTATATCATACGTTTGC | |||
| sspH1 | Host recognition/invasion (type III secretion system effector protein) | F-GGTCACAGGACACGTTCTACG | Suez et al., (2013) [35] |
| R-GCGCTTCTTCGTAATTTTCC | |||
| cdtB | Host recognition/invasion (toxin-like protein) | F-ACAACTGTCGCATCTCGCCCCGTCATT | Skyberg et al., (2006) [34] |
| R-CAATTTGCGTGGGTTCTGTAGGTGCGAGT | |||
| hlyE | Host recognition/invasion (hemolysis-inducing protein) | F-GCGTGATTGAAGGGAAATTG | Suez et al., (2013) [35] |
| R-CGAAAAGCGTCTTCTTACCG | |||
| lpfC | Host recognition/invasion (fimbrial protein) | F-GCCCCGCCTGAAGCCTGTGTTGC | Skyberg et al., (2006) [34] |
| R-AGGTCGCCGCTGTTTGAGGTTGGATA | |||
| pefA | Host recognition/invasion (fimbial protein) | F-TAAGCCACTGCGAAAGATGC | Suez et al., (2013) [35] |
| R-GCGTGAACTCCAAAAACCCG | |||
| tcfA | Host recognition/invasion (fimbrial protein) | F-TCGCTATGTTTGCATGTGGT | Suez et al., (2013) [35] |
| R-TTCAGGAACAGCCTCGAAGT | |||
| span | Entry into nonphagocytic cells (type III secretion system appratus) | F-AAAAGCCGTGGAATCCGTTAGTGAAGT | Skyberg et al., (2006) [34] |
| R-CAGCGCTGGGGATTACCGTTTTG | |||
| sipB | Entry into nonphagocytic cells (translocation machinery component) | F-GGACGCCGCCCGGGAAAAACTCTC | Skyberg et al., (2006) [34] |
| R-ACACTCCCGTCGCCGCCTTCACAA | |||
| spiA | Survival within macrophage (outer membrane secretory protein) | F-CCAGGGGTCGTTAGTGTATTGCGTGAGATG | Skyberg et al., (2006) [34] |
| R-CGCGTAACAAAGAACCCGTAGTGATGGATT | |||
| msgA | Survival within macrophage (macrophage survival protein) | F-GCCAGGCGCACGCGAAATCATCC | Skyberg et al., (2006) [34] |
| R-GCGACCAGCCACATATCAGCCTCTTCAAAC | |||
| pagC | Survival within macrophage (virulence membrane protein) | F-CGCCTTTTCCGTGGGGTATGC | Skyberg et al., (2006) [34] |
| R-GAAGCCGTTTATTTTTGTAGAGGAGATGTT | |||
| sodC | Survival within macrophage (periplasmic Cu/Zn superoxide dismutase) | F-ATGACACCACAGGCAAAACG | Suez et al., (2013) [35] |
| R-AGATGAACGATGCCCTGTCC | |||
| gatC | Growth within host (PTS galactitol transporter subunit IIC) | F-ATTGGTATCGGCTTCGTGGG | Suez et al., (2013) [35] |
| R-ATCCCCAGCCAGTATGAACC | |||
| spvB | Growth within host (ADP-ribosylating toxin) | F-CTATCAGCCCCGCACGGAGAGCAGTTTTTA | Skyberg et al., (2006) [34]. |
| R-GGAGGAGGCGGTGGCGGTGGCATCATA | |||
| sitC | Iron acquisition (permease) | F-CAGTATATGCTCAACGCGATGTGGGTCTCC | Skyberg et al., (2006) [34] |
| R-CGGGGCGAAAATAAAGGCTGTGATGAAC | |||
| iron | Iron acquisition (sidrophore) | F-ACTGGCACGGCTCGCTGTCGCTCTAT | Skyberg et al., (2006) [34] |
| R-CGCTTTACCGCCGTTCTGCCACTGC | |||
| sifA | Filamentous structure formation (secreted effector protein) | F-TTTGCCGAACGCGCCCCCACACG | Skyberg et al., (2006) [34] |
| R-GTTGCCTTTTCTTGCGCTTTCCACCCATCT | |||
| STM 2759 | Putative dipeptice/oligopetide/nikel ABC-type transport systems | F-ACCATTTTCACCTGGGCTCC | Suez et al., (2013) [35] |
| R-CGTTCAGGTTTTGTCGCTGG |