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SPR analysis of RPN13-ubiquitin and RPN13-K48-Ub2 interactions
| Ligand | Analyte | Observed KD (μm) in specified buffers |
|||
|---|---|---|---|---|---|
| HBS-TBP | HBS-TBP + RPN2 | LSPB-DBP | LSPB-DBP + RPN2 | ||
| GST-RPN13 | Ub | 109.2 ± 0.6 | 109.0 ± 0.7 | 39.8 ± 0.4 | 38.3 ± 0.4 |
| GST-RPN13(19–132) | Ub | 90.7 ± 0.6 | 93.3 ± 0.5 | 31.5 ± 0.3 | 28.9 ± 0.3 |
| GST-RPN13 | K48-Ub2 | 46.3 ± 0.2 | 68.5 ± 0.4 | 20.0 ± 0.2 | 22.6 ± 0.2 |
| GST-RPN13(19–130 | K48-Ub2 | 37.4 ± 0.2 | 64.1 ± 0.4 | 14.1 ± 0.1 | 18.9 ± 0.2 |
