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SPR analysis of RPN13 interactions with RPN2 and UCH37
| Ligand | kon (×105) | koff (×10−4) | KD (nm) |
|---|---|---|---|
| UCH37 | 1.5 ± 0.1 | 2.3 ± 0.6 | 1.7 ± 0.5 |
| UCH37 + RPN2(932–953) | 1.8 ± 0.1 | 3.2 ± 0.1 | 1.7 ± 0.1 |
| RPN2(932–953) | 9.7 ± 0.7 | 52 ± 3 | 5.4 ± 0.2 |
| RPN2(932–953) + UCH37 | 13.3 ± 0.1 | 73 ± 4 | 5.5 ± 0.4 |
