Table 1.

Multiple protein kinase inhibitors did not prevent PKD1 phosphorylation at Ser²⁰³ in IEC-18 cells

Confluent cultures of IEC-18 cells were incubated with each of the inhibitors listed below for 1 h. Then the cells were stimulated with ANG II for 60 min and lysed, and the phosphorylation of PKD1 at Ser²⁰³ was determined by immunoblotting using the antibody that detects the phosphorylated state of this residue. The inhibitors listed in this table did not prevent Ser²⁰³ phosphorylation at the concentrations tested. cPKC, conventional PKCs; nPKC, novel PKCs; aPKC, atypical PKCs; and mTOR, mechanistic target of rapamycin.

Inhibitor	Primary target	Concentration
		μm
kb NB 142–70	PKD	1–10
CRT 0066101	PKD	1–5
Go6983	cPKC, nPKC, aPKC	1–10
GF 109203X	cPKC, nPKC	3.5
KT 5720	PKA	1
KU 0063794	mTOR	1–5
GSK 690693	Akt, PKC, PKA	1–5
KU 55933	ATM	5–25
HA 1077	ROCK-II, PKA, PKG, PKCα	10
Y-27632	ROCK	10
Compound C	AMPK	5–25
Aurora kinase inhibitor III	Aurora A	10
Aurora kinase inhibitor II	Aurora A and B	10
GSK-3 inhibitor XVI	GSK-3	5
LY 294002	PI3K, mTOR	10
PP2	Src	10
AG1478	EGFR	1
U0126	MEK1/2	5
PD98059	MEK1/2	5
PIM-1 inhibitor 2	Pim-1 kinase	1–10
TG003	Clk family	1–10
Enzastaurin (LY317615)	PKCβ	1–5
GO6976	cPKC, PKD	10
KN92	CaMKII	10
KN62	CaMKII	10
FAK inhibitor 14	FAK	1–5
UCN-01	Chk1	10