Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Apr 20;292(23):9551–9566. doi: 10.1074/jbc.M116.765669

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2017 by The American Society for Biochemistry and Molecular Biology, Inc.

PMC Copyright notice

Figure 5. — Tspan5 associates with ADAM10 in mouse organs. Mouse organs were lysed, and immunoprecipitations (IP) were performed using Tspan5 or CD81 antibodies. The presence of the target antigen was analyzed by Western blotting using biotin-labeled mAbs. Because Tspan5 co-migrates with a nonspecific band, the membranes were incubated with an irrelevant biotin-labeled mAb of the same subclass (IgG2a) before incubation with the Tspan5 mAb. The presence of ADAM10 in the Tspan5 immunoprecipitates was analyzed using a polyclonal antibody to ADAM10. This experiment has been done twice with similar outcomes.