Table 1.

Kinetic parameters for wild-type and mutant CfPutA

Activity	Enzyme	Variable substrate	Km	kcat	kcat/Km
				s−1	m−1 s−1
PRODH	Wild type	Proline	145 ± 16 mm	2.6 ± 0.1	17.9 ± 0.1
	S916*	Proline			0.20 ± 0.02a
	Y927*	Proline			0.20 ± 0.02a
GSALDH	Wild type	P5C	54 ± 6 μm	1.7 ± 0.1	32,000 ± 5,000

^a Calculated from line of best fit over three data points (Fig. 3A).