Figure 2.

R565W and L640R mutations have differential effects on the surface expression of full-length versus ΔNT ADGRG1. A and C, representative Western blots showing surface and total expression of R565W and L640R mutant receptors compared with their wild-type counterparts. The lower blot in each panel represents total receptor expression, and the upper blot in each panel represents the amount of receptor pulled down by streptavidin beads (“Strep”) following biotinylation of surface-expressed proteins. B and D, quantified results of three independent Western blot experiments demonstrating that both full-length mutants exhibit markedly reduced surface and total expression, whereas ΔNT mutants do not, relative to their wild-type counterparts (one-way analysis of variance; **, p < 0.01; ***, p < 0.001 for the indicated comparisons; error bars represent S.E.). IB, immunoblotting; CT, C terminus; NS, not significant.