Figure 3.

The exposed stalk of ADGRG1 does not act as a pharmacological chaperone. A and B, to test the idea that the exposed stalk of ΔNT might act as a pharmacological chaperone to counteract surface trafficking deficits conferred by mutations to the G1 extracellular loops, a stalkless version of ΔNT-L640R (B; SL-L640R) was developed. A representative Western blot (C) and the quantified results of three independent experiments (D) demonstrate that deletion of the ΔNT-L640R stalk does not impair receptor surface expression (n = 3; error bars represent S.E.). Strep, streptavidin; IB, immunoblotting; CT, C terminus; NS, not significant.