Figure 5.

β-Arrestin2 overexpression dampens ADGRG1-mediated activation of SRF but not NFAT luciferase. A, overexpression of FLAG-tagged β-arrestin2 with full-length or ΔNT G1 resulted in significant reductions in receptor-mediated activation of SRF luciferase. B, overexpression of FLAG-β-arrestin2 full-length or ΔNT G1 had no significant effect upon ADGRG1-mediated signaling to NFAT luciferase. Results are from five independent experiments (*, p < 0.05 compared with the corresponding receptor condition without FLAG-β-arrestin2; error bars represent S.E.). luc, luciferase; NS, not significant.