. 2017 Apr 25;292(23):9733–9744. doi: 10.1074/jbc.M117.788554

Table 1.

Summary of ceCblC-catalyzed GSH-dependent cobalamin processing and GSSG production rates

Data represent the mean ± S.D. of at least three independent experiments.

Cobalamin	k_Cbl^a	k_GSSG^b
	min⁻¹	min⁻¹
CNCbl	0.046 ± 003^c	2.9 ± 0.1
AdoCbl	0.19 ± 0.03^d	19 ± 2
OH₂Cbl^e	0.46 ± 0.02	21 ± 2
MeCbl	13.5 ± 0.4^d	83 ± 9
GSCbl	108 ± 12	102 ± 9

^a Cobalamin processing rates were determined by mixing ceCblC (40 μm)-cobalamin (20 μm) with 4 mm GSH in Buffer A under anaerobic conditions at 20 °C. The initial rates of the reactions were determined. With GSCbl, a single exponential fit was used to obtain the k_obs.

^b GSSG production rate was determined using ceCblC (1–10 μm)-cobalamin (0.5–5 μm) complex in the presence of 4 mm GSH under aerobic conditions.

^c From Ref. 12.

^d From Ref. 13.

^e ceCblC-OH₂Cbl complex was generated by oxidation of ceCblC-cob(II)alamin in the presence of the xanthine/xanthine oxidase system followed by rigorous washing to remove xanthine.