FIG. 11.
Repression or inactivation of F10 alters the phosphoprotein profile of infected cells. Cells were infected (MOI = 5) with either vtetR (lane 1), ts28 (lane 2), or vΔiF10 in the absence of TET (lane 3) at 40°C and were metabolically labeled with 32PPi from 4 to 8 hpi. Cytoplasmic extracts were fractionated in an SDS-15% PAGE gel and analyzed by autoradiography. While several phosphorylated proteins were found to decrease in intensity during nonpermissive vΔiF10 and ts28 infections (↓), the more striking result was the appearance of prominent phosphorylated proteins (↑) under these conditions (compare lanes 2 and 3 to lane 1). The migration of protein standards is shown on the left, with molecular masses indicated in kilodaltons.