FIG. 3.

OTI CD8⁺ T cells control lethal infection by γHV68.OVA. (A) Lethality from wild-type γHV68 infection is identical in both RAG and OTI/RAG mice, whereas lethality from γHV68.OVA is significantly delayed in OTI/RAG mice after i.p. (4 × 10³ PFU) infection (P < 0.0001). (B) Similar results to those in panels A were obtained after intranasal infection with 400 PFU. (C) Significant reductions in lethality were seen after OTI T-cell transfer to RAG mice, both with total splenocytes from OTI/B6 (OTI spl) versus B6 splenocytes (B6 spl) (P < 0.005) and with CD8⁺ T cells enriched from OTI/B6 splenocytes (OTI/CD8) versus CD8⁺ T cells enriched from B6 splenocytes (B6/CD8) (P < 0.005). These data are representative of between two and four experiments for each condition. n, number of mice.