FIG. 4.

Antigen-specific activation of OTI CD8⁺ T cells in γHV68.OVA-infected mice. (A) PBL and splenocytes were isolated from naïve OTI/RAG mice or from OTI/RAG mice infected with γHV68.OVA (10⁴ PFU i.p.) for 14 or 22 days. The cells were stained for cell surface expression of CD8α and Vα2. (B) Equal numbers of OTI and 2C CD8⁺ T cells were coinjected into RAG recipients, which were then infected with γHV68.OVA. CD8⁺ T cells were evaluated 16 days later for cell surface expression of activation markers and for increased granularity. The plots are side scatter by either cell surface molecule or intracellular IFN-γ and are gated on CD8⁺ (for 2C T cells) or CD8⁺/Vα2⁺ (for OTI T cells). The circles highlight OTI T cells that have both increased granularity and levels of surface marker or IFN-γ consistent with activation. These data are representative of two independent experiments.