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. 2017 Jun 8;16:99. doi: 10.1186/s12934-017-0715-8

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© The Author(s) 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Strain phenotypic analysis. Strains LK and M12 were grown in different media to confirm drug resistance and leucine assimilation. X-33 was used as a control. a YPD containing G418 or hygromycin B (Hyg B). b MD lacking or not leucine in different concentrations. c MD with leucine replacing ammonium sulfate. d Buffered MD (pH 6.0) containing leucine. X-33 wild-type strain with intact LEU2 gene, LK leu2 strain disrupted with kan cassette, M12 strain obtained after marker removal with CreA recombinase