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. 2017 Jun 8;16:99. doi: 10.1186/s12934-017-0715-8

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© The Author(s) 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Heterologous expression in K. phaffii M12. Intracellular expression of enhanced green fluorescent protein (EGFP) was visualized by epifluorescence microscopy. Untransformed (M12) and transformed (M12 + GFP) cells were viewed under differential interference contrast (DIC) or fluorescence (FM) microscopy after growth in glycerol or methanol containing media. All images were collected with the same exposure time