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. 2017 Jun 8;16:99. doi: 10.1186/s12934-017-0715-8

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© The Author(s) 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — Vector copy number determination. a Schematic representation of genomic contexts and expected sizes of bands obtained after hybridization with a PGK probe (annealing positions are represented by a red line). Dark grey areas correspond to integrated P_PGK1 sequences derived from vector. b Result of Southern blot analysis. c Correlation between copy number and maximum growth rates of selected clones. Error bars represent standard error of the mean. M O’GeneRuler 1 kb DNA ladder, M12 K. phaffii leu2 strain, E EcoRI restriction site