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. 2017 Jun 8;16:99. doi: 10.1186/s12934-017-0715-8

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© The Author(s) 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 6 — Intracellular EGFP production. a Flow cytometry analysis of the cells positive for EGFP production. b Correlation between copy number and EGFP production. M12 leu2 strain. Error bars represent standard error of the mean. Asterisks indicate significant difference between the evaluated clone and the M12 control strain according to ANOVA followed by Tukey’s post-test (p < 0.05)