Table 2.
Primers used in this work
| Primer | Sequence (5′→3′)* | Restriction site |
|---|---|---|
| PpLEU2-F1 | CAGCTGAAGAGTCCAAGTCCAAG | PvuII |
| PpLEU2-R2 | CAGCTGGTGCCATTGGTGGTACTGT | PvuII |
| TEF-2F | ATACCTAGGCCCCACACACCATAGCTTCAA | AvrII |
| TEF-2R | ATACCTAGGTTTGTAATTAAAACTTAGATTAGATTG | AvrII |
| ZeoBlas-F3 | CGGATCCATAACTTCGTATAATGTATGCTATACGAAGTTATAGATCTCCCACACACCATAGCTTCAAAATG | BamHI and BglII |
| ZeoBlas-R3 | CGGATCCATAACTTCGTATAGCATACATTATACGAAGTTATAGATCTAGCTTGCAAATTAAAGCCTTCGAG | BamHI and BglII |
| PpLEU2-EXT1 | GAGGATAAGCTGGGAGACTATG | – |
| PpLEU2-EXT2 | TCTGTTGCCTAAGACTGAGAGC | – |
| 5-leud | GAGATCTATATATATTTCAAGGATATACCATTCTAATG | BglII |
| 3-leud | GAGATCTGTTTCATGATTTTCTGTTACACC | BglII |
* Restriction sites are underlined