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Hyperphosphorylation of IRF-3. Extracts from cells infected for 8 h (a) or 16 h (b) were analyzed by sodium dodecyl sulfate gel electrophoresis, followed by immunoblot analyses to detect IRF-3 (panels 1), the N protein of SARS-CoV (panels 2), or the N protein of the control virus (panels 3). The two forms of unphosphorylated IRF-3 are indicated by arrowheads, and the hyperphosphorylated form of IRF-3 is indicated by an asterisk (*).
