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Mutations in cysteine residues of gN do not prevent recognition by MAb 14-16A. Cos7 cells were transfected with the indicated plasmids, and protein expression was assayed by reactivity with MAb 14-16A (anti-gM/gN) and MAb 9E10 (anti-Myc). Reactivity was detected with an anti-mouse • • chain-specific antibody. MAb 9E10 was directly coupled with Cy3. The appearance of yellow in the merged pictures indicates colocalization of signals.
