FIG. 3.

Cysteine 90 of gN and cysteine 44 of gM are involved in disulfide linkage in the gM/gN complex. 293T cells were transfected with gM and the gN mutant plasmids (A) or gN and the gM mutant plasmid (B). Cell lysates were subjected to immunoprecipitation with Myc-specific MAb 9E10. SDS-PAGE was performed in the presence (+) or absence (−) of the reducing agent 2-mercaptoethanol (2-ME). Proteins were detected in Western blots with MAb 14-16A (anti-gN) (A) or MAb 14-16A and the anti-gM MAb IMP 91-3/1 (22) (B). The sizes are indicated on the right of each panel.