TABLE 1.
gM carboxyl-terminal truncation mutants and complex formation
| gM | Amino acida changed | Predicted TM domainb | Reactive with MAb 14-16Ac |
|---|---|---|---|
| gM-2 | 118 | 119-141 | Yes |
| gM-3 | 150 | 151-173 | Yes |
| gM-5 | 200 | 201-223 | Yes |
| gM-6 | 224 | 201-223 | Yes |
| gM-7 | 236 | 238-260 | Yes |
| gM-8 | 262 | 238-260 | Yes |
| gM-11 | 298 | 299-321 | Yes |
| gM-12 | 322 | 299-321 | Yes |
| gM-wt | 358 | Yes |
a
Amino acids numbered according to HCMV strain AD169.
b
The predicted transmembrane domains were generated with the TMHMM server http://www.cbs.dtu.dk/services/TMHMM. The predicted topology of gM is that the amino terminus of the molecule lies within the lumen (extracytoplasmic) and the carboxyl terminus of the molecule (321 to 358) is cytoplasmic.
c
Reactivity was determined in indirect immunofluorescence following cotransfection of the indicated gM plasmids together with a wild-type gN-expressing plasmid.