FIG. 3.

Impaired inducibility of composite CD28 responsive element in cells expressing HCV C. Jurkat (Jk) and C-expressing cell lines (JHC.d, JHC.g, and JHC.h) were transfected with reporter plasmids containing either three distal composite NFAT elements or four composite CD28 responsive elements linked to the luciferase gene. At 40 h posttransfection, the cells were stimulated with TPA and ionomycin for 6 h, and the relative luciferase activities were determined. For each reporter gene, all activities were normalized to those of stimulated control cells. Values are means ± SEM of three independent experiments.