FIG. 5.

Activation of MAP kinases ERK1/2 and p38 in HCV C-expressing cells. Phosphorylated ERK1/2 and p38 in control cells (Jk) and HCV C-expressing cells (JHC.d, JHC.g, and JHC.h) were detected in Western blots by the use of phosphospecific antibodies to ERK1/2 and p38. Where indicated, the cells were stimulated with TPA and ionomycin (Iono) for 20 min. The expected positions of the two isoforms of ERK1/2 (42 and 44 kDa) and p38 are indicated to the left. The positions of molecular size markers (in kilodaltons) run in parallel are indicated to the right. The data presented are representative of three independent experiments.