TABLE 2.
Detection of neutralizing antibodies in serum and SARS-CoV in postmortem tissues
| Group (virus isolate) | Animal no. | Time of euthanasia (dpi) | Neutralizing antibody titera | Detection in postmortem tissuesc
|
|||||
|---|---|---|---|---|---|---|---|---|---|
| Lung | Heart | Spleen | Lymph node | Kidney | Liver | ||||
| A (GZ01) | 6 | 3 | NDb | +/+ | −/− | −/+ | +/+ | +/+ | +/+ |
| 5 | 13 | 20 | −/− | −/+ | −/+ | −/ND | −/− | −/− | |
| 14 | 23 | 80 | −/− | −/− | −/+ | −/+ | −/− | −/− | |
| 4 | 34 | 40 | −/− | −/− | −/+ | −/+ | −/− | −/− | |
| 3 | 35 | 40 | −/− | −/− | −/− | −/+ | −/− | −/− | |
| B (BJ01) | 13 | 3 | <10 | +/+ | +/+ | +/+ | +/+ | +/+ | +/+ |
| 7 | 13 | 20 | −/− | −/− | −/+ | −/+ | −/+ | −/+ | |
| 11 | 23 | 80 | −/− | −/− | −/+ | −/+ | −/− | −/− | |
| 10 | 34 | 80 | −/− | −/− | −/+ | −/+ | −/− | −/− | |
| 8 | 35 | 80 | −/− | −/− | −/− | −/+ | −/− | −/− | |
a
All animals were tested prior to inoculation, and none of the animal had neutralizing antibodies to SARS-CoV in their prebleed sera.
b
ND, not determined.
c
Virus isolation/RT-PCR results are depicted. +/+ indicates positive for both virus isolation and PCR, whereas −/+ means that particular tissue gave positive PCR signals but virus isolation was negative.