TABLE 2.
LCMV and PRH expression in lethally infected monkey livers
| Monkeya | Presence of viral RNA in liverb | Fold inhibition of PRH mRNAc |
|---|---|---|
| Rh-1a-Ctrl | − | 1 |
| Rh-1b-Ctrl | − | 1 |
| Rh.iv3-d1a | − | 2.0 |
| Rh.iv3-d1b | − | 2.3 |
| Rh.iv3-d2a | − | 3.3 |
| Rh.iv3-d2b | − | 2.5 |
| Rh.iv3-d3a | − | 1.6 |
| Rh.iv3-d4a | − | 3.0 |
| Rh.iv3-d4b | + | 2.5 |
| Rh.iv3-d6a | + | 2.7 |
| Rh.iv3-d6b | + | 3.9 |
| Rh.iv3-d7a | + | 4.2 |
| Rh.iv3-d11 | + | 3.5 |
| Rh.iv6-d12 | + | 4.3 |
Ctrl is a mock-infected control animal; d1 is 1 day after infection. Rh.iv3-d11 and Rh.iv6-d12 were two monkeys that were lethally infected with 103 or 106 PFU of virus and then euthanized on day 11 or 12, respectively. Although these two animals were previously described (37, 38), the remaining animals designated Rh.iv3 have not been described. They were also healthy rhesus macaques that were intravenously infected with 103 PFU of virus and then sacrificed on different days after infection (d2, d3, d4, d6, or d7), as indicated by their names.
+, positive by RT-PCR for viral RNA; −, negative by RT-PCR for viral RNA.
Fold inhibition, or relative quantitation (RQ = 2−ΔΔCT), is a quantitative measurement of real-time PCR mRNA levels in comparison with a standard. We used GAPDH and 18S RNA standards and gave the values here for GAPDH standardization, which was less dramatic and more reliable than 18S standardization.