FIG. 6.

Effect of RNA polymerase II inhibitors. At 9 hpi of CEF with avian reovirus (A) and 24 h after the transfection of Vero cells with the pCINeo plasmid (B), the cells were incubated as follows: (1), 5 h at 37°C with 20 μg of cycloheximide/ml; (2), 5 h at 37°C with 20 μg of cycloheximide/ml and 100 μM DRB; (3), 5 h at 4°C with 20 μg of cycloheximide/ml and 100 μM DRB; and (4), 5 h at 37°C with 20 μg of cycloheximide/ml and 100 μM DRB, followed by another 5-h incubation at 37°C with 20 μg of cycloheximide/ml. At the end of the incubation period, the cells were fixed, stained with DAPI, and immunostained for p17 (FITC). The stained cells were visualized by fluorescence microscopy.