FIG. 7.

Subcellular fractionation. At 9 hpi, mock-infected (U) (lanes 1 and 2) and infected (I) (lanes 3 and 4) CEF cells were mock treated (A, C, and D) or treated for 5 h at 37°C with 20 μg of cycloheximide/ml and 100 μM DRB (B). The cells were then lysed, and the lysates were fractionated into cytoplasmic (lanes 1 and 3) and nuclear (lanes 2 and 4) fractions as described in Materials and Methods. The fractions were analyzed by Western blotting with specific antibodies against p17, nucleolin, and tubulin, as indicated on the right.