FIG. 1.

HSP expression in persistently BDV-infected glial cells. (A) Morphology and BDV antigen expression in persistently BDV-infected glial cell lines. The C6 (a to d) and OL (e to h) cell lines were grown under normal culture conditions. Subpanels: a, b, e, and f, uninfected; c, d g, and h, persistently infected. The cells were fixed and stained with hematoxylin. BDV antigen was detected by immunofluorescence assay with an anti-P polyclonal antibody. (B) Proliferation of persistently infected glial cells. Cells (2 × 10⁵) were seeded in 60-mm-diameter culture plates, and their proliferation was evaluated every 12 h. (C) Expression of large HSPs in persistently infected cell lines. Cells were lysed, and equal amounts of protein were subjected to Western blot analysis with antibodies against HSP90, HSP70, HSC70, HSP60, and BDV P and N. The GAPDH control shows that each lane contains approximately the same amount of protein. Data from one experiment representative of three independent tests are shown.