FIG. 2.

BDV infection inhibits HSP70 expression induced by heat shock in glial cells. (A) C6 and OL cells were exposed to heat shock at 44°C and allowed to recover at 37°C for different periods. Cells were harvested at the indicated times during the recovery period. Nontreated control cells (NT) were maintained at 37°C and harvested together with heat-stressed cells. After being washed with ice-cold PBS, the cells were lysed and separated by SDS-PAGE (10% acrylamide). Western blot analysis was carried out as described in Materials and Methods. Data from one experiment representative of six independent tests are shown. (B) For quantitative analysis of HSP expression, band intensities were determined with NIH Image software. Values were normalized to GAPDH levels.