FIG. 6.

Induction of autophosphorylated PKR in BDV-infected glial cells during heat stress. (A) After heat shock, uninfected and BDV-infected C6 cells were washed with ice-cold PBS and lysed in lysis buffer. Nontreated control cells (NT) were maintained at 37°C and harvested together with heat-stressed cells. Equivalent amounts of proteins were separated by SDS-PAGE (10% acrylamide). Western blot analysis was carried out to examine relative levels of phosphorylated PKR (P-PKR). Data from one experiment representative of three independent tests are shown. (B) For quantitative analysis, band intensities were determined with NIH Image software. Values were normalized to GAPDH levels. The data are expressed as the mean plus the standard error.