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Ad-mediated disruption of model membranes (liposomes) containing an entrapped fluorophore. (A) Ad5 particles (75 μg/ml) were incubated with liposomes (2 μg/ml) at different pH levels, and the release of SulfoB was monitored over time as described in Materials and Methods. (B) The amount of SulfoB released from liposomes incubated with Ad5 or ts1 particles was measured at different pHs, following preincubation of virions at 25 or 45°C (for WT) or 55°C (for ts1).
