FIG. 6.
Schematic representation of Ad protein VI. Sites for cleavage by the 23K cysteine protease, hexon binding, and nuclear export (NES1) and import (nuclear localization signal 2 [NLS2]) are indicated. A predicted amphipathic α-helical domain is located between residues 36 to 53. A helical wheel diagram of this region is shown with the hydrophobic residues in black and the hydrophilic residues in white. An alignment of protein VI sequences corresponding to the amphipathic helical domain of human and nonhuman Ads is shown. Predicted ΔGinterfacial values for the partitioning of these domains into lipid membranes are provided for comparison.