FIG. 7.

Analysis of the membrane lytic activity recombinant protein VI molecules. (A) SDS-PAGE of recombinant preprotein VI, protein VI, and a truncated protein VI (VIΔ54), following purification on nitriloacetic acid-Ni²⁺ agarose columns. (B) Liposome disruption by equivalent amount of pVI ♦, VI ▪, VIΔ54 ▴, or BSA • as a control was assessed at various pH levels by SulfoB release. (C) EMs of untreated liposomes (i) or liposomes treated with an equivalent weight of protein VI at pH 7.4 (ii) obtained at a magnification of ×21,000 after being negatively stained with 2% phosphotungstate. Bar, 500 nm.