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. 2017 Apr 21;129(23):3074–3086. doi: 10.1182/blood-2016-09-740209

Figure 2.

Figure 2.

Expression of miR-155 in FLT3-ITD BM promotes expansion of myeloid progenitors. (A) Expression level of miR-155 in WT, 155−/−, FLT3-ITD, and FLT3-ITD 155−/− mice in total BM determined by qRT-PCR. Expression normalized to 5S ribosomal RNA. (B) Total BM cellularity in different mouse groups. (C) Total numbers of CD11b+ Gr1+ cells, CD11b+ Gr1– cells, B220+ cells, and Ter119+ cells in the BM of different mouse groups determined by flow cytometry. (D) Gating strategy for flow cytometric analysis of LKS and myeloid progenitor (Lin–, c-Kit+, Sca1–) populations. (E) Total number of LKS cells in BM determined by flow cytometry. (F) Total number of myeloid progenitor cells (Lin–, c-Kit+, Sca1–) in BM determined by flow cytometry. (G) Total common myeloid progenitor (CMP) (left), granulocyte-monocyte progenitor (middle), and megakaryocyte-erythrocyte progenitor (right) cells in BM determined by flow cytometry. Data are representative of at least 3 independent experiments. Each point represents a sample from 1 mouse. *P ≤ .05; **P ≤ .01; ***P ≤ .001; ****P ≤ .0001; ns, P > .05.