TABLE 2.
Primer sequences positive for Bat-CoV detection
| PCR | Target sequence | Primer orientation | Primer sequence (5′ to 3′) |
|---|---|---|---|
| 1 | RdRp | Forward | AYAACCAAGATCTTAATGG |
| Reverse | TGCTTAGAACCCAAAATCAT | ||
| 2a | RdRp | Forward | GGTTGGGACTATCCTAAGTGTGA |
| Reverse | CCATCATCAGATAGAATCATCATA | ||
| 3 | Helicase-ExoN | Forward | CTCARGGTAGTGARTATGA |
| Reverse | AATTGTTCWCCWGGTGG | ||
| 4 | Spike | Forward | WTATGTTTGYAATGGTAAY |
| Reverse | GTCWTCATCMACWGTRC | ||
| 5 | Spike | Forward | GAYTDDCAGCACTTAATGC |
| Reverse | TTGAGCCAYTCAAGRTYRA | ||
| 6 | Spike | Forward | CAATCTAGGTCTGCTATCG |
| Reverse | CTAGAAGACTGTGATTTGA |
a
Forward and reverse primers (IN-6 and IN-7) were communicated through the World Health Organization's SARS etiology network by colleagues from the Centers for Disease Control and Prevention.