Table 1.

Recipes for preparing zymogram separating gels with different percentages of acrylamide. The volumes are sufficient for a single mini-gel (9 cm × 6 cm × 0.75 cm).

Solution	Final acrylamide concentration in gel
	6%	7.5%	10%	12%
Acrylamide/bisacrylamide solution 37.5:1	0.8 mL	1.0 mL	1.32 mL	1.61 mL
Separating gel buffer	1.0 mL	1.0 mL	1.0 mL	1.0 mL
10X casein or gelatin substrate solution	0.4 mL	0.4 mL	0.4 mL	0.4 mL
Sucrose solution	0.86 mL	0.86 mL	0.86 mL	0.86 mL
Water	0.94 mL	0.74 mL	0.42 mL	0.13 mL
10% (w/v) ammonium persulfate	14 μL	14 μL	14 μL	14 μL
TEMED*	1.5 μL	1.5 μL	1.5 μL	1.5 μL

^*TEMED: N,N,N′,N′-tetramethylethylenediamine.