FIG. 2.
Flow cytometric analysis of DC after amplicon transduction. DC transduced in vitro with HSV gp120MN/LAI amplicons (MOI, 1) showed abundant surface expression of gp120 as detected by flow cytometry with gp120-specific Ab. Sixty percent of HLA-DR-positive cells expressed HIV-1 gp120. Increased expression of HLA-DR, costimulatory molecules (CD80 and CD86), and the maturation marker (CD83) was detected after HSV transduction compared to that of nontransduced DC. Surface expression of these antigens was analyzed 48 h posttransduction. Appropriate isotype controls were used to set the gates. The expression of surface markers upon HSV amplicon transduction was analyzed in at least four individual experiments using cells from different donors. The results shown are from a representative experiment.