FIG. 6.

Protection of human CD4⁺ T cells following HIV-1 challenge. Mice were immunized with HSV-transduced DC (HSV gp120_MN/LAI or HSVlac in the vaccinated and control groups, respectively). Seven days later, the animals were challenged with infectious HIV-1_LAI. Splenocytes were harvested 14 days after challenge and subjected to immunophenotypic analysis, using monoclonal Abs specific for human CD4 and CD8. (A) Flow cytometric analysis. Pooled splenocytes from six to eight mice per group were stained for CD4⁺ and CD8⁺ T cells. (B) Bar graph representing the mean ± SEM values for CD4⁺ and CD8⁺ human T cells, obtained from the control and vaccinated mice challenged with LAI (six to eight animals per group). *, P < 0.05 by Student's t test.