Table S3.

Metrics obtained from stochastic simulations and their variation due to changes in the initial conditions

Parameter	Value*	Median fragment length, nt	Median gap length, nt	Median initiation time, s	DNA replicated†	Primer utilization, %	Collision, %
Holoenzyme procession rate, nt⋅s−1	300	2,250	2,194	6.2	80.1	11.0	70.0
	150	1,893	2,919	5.9	66.2	8.8	63.8
	75	2,833	6,917	5.7	56.2	2.9	68.6
	30	2,968	12,491	5.6	33.9	1.1	82.1
	15	1,845	22,051	5.0	14.9	0.7	89.8
Priming rate, s−1	0.750	1,893	2,919	5.9	66.2	8.8	63.8
	0.375	3,441	5,273	10.3	70.5	7.8	70.5
	0.150	9,854	13,476	22.8	78.9	7.1	82.6
	0.075	19,048	23,544	40.4	84.7	7.8	90.3
Clamp/clamp loader concentration, molecules	1,400	1,439	1,860	2.7	71.3	13.3	67.9
	700	1,893	2,919	5.9	66.2	8.8	63.8
	350	5,410	4,859	12.4	60.1	5.5	59.9
	140	3,101	9,585	29.8	52.3	2.7	57.7

^*The default value in each case is shown in bold face.

^†The percentage of DNA synthesis was calculated as the ratio between the number of bases synthesized and the sum of the total bases synthesized and total gaps. This is fundamentally a different measure and inherently lower than the total number of bases unwound by the helicase.