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TMV helicase interactions. (A) Quantification of β-galactosidase activity between PAP1 and ETR1, a noninteracting control protein; PAP1 and LEXA, the empty vector; PAP1 and WT-TMV HEL, encoding the helicase domain of the viral replicase; PAP1 and TMV-V1087I HEL, a mutant TMV helicase; and IAA10, an Aux/IAA family member, with WT-TMV HEL. (B) Western immunoblot assay comparing the accumulation of WT-TMV HEL and TMV-V1087I HEL proteins in yeast.
