FIG. 1.

MOI dependence of reovirus-induced host shutoff. L929 cells (A) or wt MEFs (B) were mock infected or infected in duplicate at a variety of MOIs with the indicated strains of reovirus. At 20 h p.i., cells were pulse-labeled with [³⁵S]methionine-cysteine for 30 min, extracts were prepared, and proteins were separated by SDS-PAGE. Cellular translation was quantified as described in the legend to Fig. 2. The graphs depict the average percent cellular translation relative to that in mock-infected cells; error bars indicate deviations from the average.